The ability of the yeast Saccharomyces cerevisiae to enter meiosis and sporulate is conferred by the a1-alpha 2 regulatory activity of the mating-type locus. We have cloned the RME1 gene and show that: the RME1 transcript is repressed by a1-alpha 2 in a/alpha cells; overexpression of the RME1 product in a/alpha cells blocks sporulation; and inactivation of RME1 by a null mutation constructed in vitro permits cells lacking a1-alpha 2 activity to sporulate. The RME1 product thus inhibits meiosis; the mating-type locus permits entry into meiosis by repression of RME1.

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a1alpha 2 regulatory activity matingtype locus rme1 gene

A P, Mitchell I, Herskowitz,.Activation of meiosis and sporulation by repression of the RME1 product in yeast.. 319 (6056),738-42.

A P, Mitchell I, Herskowitz,"Activation of meiosis and sporulation by repression of the RME1 product in yeast." 319

A P, Mitchell I, Herskowitz,"Activation of meiosis and sporulation by repression of the RME1 product in yeast."