Peg3 (Paternally Expressed Gene 3) is an imprinted gene that encodes a zinc finger DNA-binding protein. Peg3 itself is localized in the middle of a KRAB-A (Kruppel-Associated Box) zinc finger gene cluster. The amino acid sequence encoded by its exon 7 also shows sequence similarity to that of KRAB-A, suggesting Peg3 as a KRAB-containing zinc finger gene. As predicted, the PEG3 protein was co-immunoprecipitated with KAP1, a co-repressor that interacts with KRAB-A. A series of follow-up experiments further demonstrated that the exon 7 of PEG3 is indeed responsible for its physical interaction with KAP1. ChIP and promoter assays also indicated that PEG3 likely controls its downstream genes through the KAP1-mediated repression mechanism. Overall, the current study identifies PEG3 as a KRAB-containing zinc finger protein that interacts with the co-repressor protein KAP1.

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kraba kruppelassociated box zinc finger gene expressed imprinted gene corepressor protein kap1 peg3 kap1mediated repression mechanism

Hongzhi He,An Ye,Hana Kim,Joomyeong Kim,.PEG3 Interacts with KAP1 through KRAB-A.. 11 (11),.

Hongzhi He,An Ye,Hana Kim,Joomyeong Kim,"PEG3 Interacts with KAP1 through KRAB-A." 11

Hongzhi He,An Ye,Hana Kim,Joomyeong Kim,"PEG3 Interacts with KAP1 through KRAB-A."