As increases in hepatocyte growth factor/scatter factor (HGF/SF) induce retinal pigment epithelial (RPE) migration and proliferation into the vitreous cavity and contribute to proliferative vitreoretinopathy (PVR) development, we determined if changes in miR-182 expression affect such behavioral changes. We found that miR-182 expression was less in PVR clinical samples than in primary RPE cells whereas c-Met was upregulated. Ectopic miR-182 inhibited RPE cell proliferation, cell cycle, and migration. Bioinformatic analysis identified c-Met as a miR-182 target, which was confirmed with the luciferase reporter assay. Transfection of miR-182 into RPE cells induced c-Met downregulation, which led to reduced cell proliferation and migration through declines in p-Akt formation. MiR-182 downregulation along with c-Met upregulation in PVR tissues suggest that these two opposing effects play important roles in PVR development. As ectopic miR-182 expression suppressed RPE cell proliferation and migration, strategies to selectively upregulate miR-182 expression in a clinical setting may provide a novel option to treat this disease.

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luciferase reporter retinal pigment epithelial rpe pakt cycle hepatocyte growth factorscatter factor hgfsf mir182 expression proliferative vitreoretinopathy pvr cmet cell proliferation migration

Lihua Wang,Feng Dong,Peter S Reinach,Dandan He,Xiaoting Zhao,Xiaoyan Chen,Dan-Ning Hu,Dongsheng Yan,.MicroRNA-182 Suppresses HGF/SF-Induced Increases in Retinal Pigment Epithelial Cell Proliferation and Migration through Targeting c-Met.. 11 (12),.

Lihua Wang,Feng Dong,Peter S Reinach,Dandan He,Xiaoting Zhao,Xiaoyan Chen,Dan-Ning Hu,Dongsheng Yan,"MicroRNA-182 Suppresses HGF/SF-Induced Increases in Retinal Pigment Epithelial Cell Proliferation and Migration through Targeting c-Met." 11

Lihua Wang,Feng Dong,Peter S Reinach,Dandan He,Xiaoting Zhao,Xiaoyan Chen,Dan-Ning Hu,Dongsheng Yan,"MicroRNA-182 Suppresses HGF/SF-Induced Increases in Retinal Pigment Epithelial Cell Proliferation and Migration through Targeting c-Met."