The type VI secretion system (T6SS) is a nanomachine deployed by many Gram-negative bacteria as a weapon against eukaryotic hosts or prokaryotic competitors. It assembles into a bacteriophage tail-like structure that can transport effector proteins into the environment or target cells for competitive survival or pathogenesis. T6SS effectors have been identified by a variety of approaches, including knowledge/hypothesis-dependent and discovery-driven approaches. Here, we review and discuss the methods that have been used to identify T6SS effectors and the biological and biochemical functions of known effectors. On the basis of the nature and transport mechanisms of T6SS effectors, we further propose potential strategies that may be applicable to identify new T6SS effectors.

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discoverydriven mechanisms bacteriophage taillike structure effector proteins vi secretion system t6ss biological and biochemical knowledgehypothesisdependent t6ss effectors

Yun-Wei Lien,Erh-Min Lai,.Type VI Secretion Effectors: Methodologies and Biology. 7 (),.

Yun-Wei Lien,Erh-Min Lai,"Type VI Secretion Effectors: Methodologies and Biology" 7

Yun-Wei Lien,Erh-Min Lai,"Type VI Secretion Effectors: Methodologies and Biology"