BACKGROUND: Understanding the early relationship between brain tumor cells and their environment could lead to more sensitive biomarkers and new therapeutic strategies. We have been using a rodent model of neurocarcinogenesis in which all animals develop brain tumors by six months of age to establish two early landmarks in glioma development: the appearance of a nestin(+) cell at thirty days of age and the appearance of cellular hyperplasia between 60 and 120 days of age. We now report an assessment of the CSF proteome to determine the changes in protein composition that occur during this period. MATERIALS AND METHODS: Nestin(+) cell clusters and microtumors were assessed in 63 ethylnitrosourea-exposed rats on 30, 60, and 90 days of age. CSF was obtained from the cisterna magna from 101 exposed and control rats at 30, 60, and 90 days and then analyzed using mass spectrometry. Differentially expressed peaks were isolated and identified. RESULTS: Nestin(+) cells were noted in all ethylnitrosourea-exposed rats assessed pathologically. Small microtumors were noted in 0%, 18%, and 67% of 30-, 60-, and 90-day old rats, respectively (p

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nestin cell cell clusters and microtumors brain tumor cells protein composition cellular hyperplasia csf proteome neurocarcinogenesis differentially expressed peaks

John C Whitin,Taichang Jang,Milton Merchant,Tom T-S Yu,Kenneth Lau,Benjamin Recht,Harvey J Cohen,Lawrence Recht,.Alterations in cerebrospinal fluid proteins in a presymptomatic primary glioma model.. 7 (11),.

John C Whitin,Taichang Jang,Milton Merchant,Tom T-S Yu,Kenneth Lau,Benjamin Recht,Harvey J Cohen,Lawrence Recht,"Alterations in cerebrospinal fluid proteins in a presymptomatic primary glioma model." 7

John C Whitin,Taichang Jang,Milton Merchant,Tom T-S Yu,Kenneth Lau,Benjamin Recht,Harvey J Cohen,Lawrence Recht,"Alterations in cerebrospinal fluid proteins in a presymptomatic primary glioma model."