A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%). High correlation of measured parasitaemia (r=0.98, P

+More

parasitaemia cultures flow cytometric facs detection method stained microscopic examination precision of parasitemia falciparum

Chae Seung Lim,Jin Woo Jang,Ju Yeon Kim,Jung Yoon,Soo Young Yoon,Chi Hyun Cho,Eun Taek Han,Seong Soo A. An,.Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A. 2014 (),.

Chae Seung Lim,Jin Woo Jang,Ju Yeon Kim,Jung Yoon,Soo Young Yoon,Chi Hyun Cho,Eun Taek Han,Seong Soo A. An,"Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A" 2014

Chae Seung Lim,Jin Woo Jang,Ju Yeon Kim,Jung Yoon,Soo Young Yoon,Chi Hyun Cho,Eun Taek Han,Seong Soo A. An,"Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A"