The occurrence of bacterial endosymbionts in free-living amoebae has been known for decades, but their obligate intracellular lifestyle hampered their identification. Application of the full cycle rRNA approach, including 16S rRNA gene sequencing and fluorescence in-situ hybridization with 16S rRNA-targeted oligonucleotide probes, assigned the symbionts of Acanthamoeba spp. and Hartmannella sp. to five different evolutionary lineages within the Proteobacteria, the Bacteroidetes, and the Chlamydiae, respectively. Some of these bacterial symbionts are most closely related to bacterial pathogens of humans, and it has been suggested that they should be considered potential emerging pathogens. Complete genome sequence analysis of a chlamydia-related symbiont of Acanthamoeba sp. showed that this endosymbiont uses similar mechanisms for interaction with its eukaryotic host cell as do the well-known bacterial pathogens of humans. Furthermore, phylogenetic analysis suggested that these mechanisms have been evolved by the ancestor of these amoeba symbionts in interplay with ancient unicellular eukaryotes.

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pathogens 16s rrnatargeted oligonucleotide fluorescence insitu hybridization humans chlamydiarelated symbiont of acanthamoeba unicellular eukaryotes rrna gene sequencing endosymbionts full cycle rrna approach genome sequence symbionts freeliving amoebae

Matthias, Horn Michael, Wagner,.Bacterial endosymbionts of free-living amoebae.. 51 (5),509-14.

Matthias, Horn Michael, Wagner,"Bacterial endosymbionts of free-living amoebae." 51

Matthias, Horn Michael, Wagner,"Bacterial endosymbionts of free-living amoebae."