K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double-stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neurtal M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with the K2 neutral M genome. Hybridization showed that the USM12 M dsRNA had significant homology with the K2 M dsRNA. Protein profiles of extracellular proteins from USM12 and a cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.

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denatured doublestranded rna dsrna reannealed k2 neurtal m dsrna killer yeast m inverted duplication

B D, Wingfield V J, Southgate I S, Pretorius H J, van Vuuren,.A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome.. 6 (2),159-69.

B D, Wingfield V J, Southgate I S, Pretorius H J, van Vuuren,"A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome." 6

B D, Wingfield V J, Southgate I S, Pretorius H J, van Vuuren,"A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome."