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Experimental Cell Research | Vol.15, Issue.2 | | Pages 358-369

Experimental Cell Research

Surface antigen defects contributing to developmental failure in aggregateless variants of the slime mold, Dictyostelium discoideum

J.H. Gregg and C.W. Trygstad  
Abstract

1. 1. The procedure for obtaining antisera to wild type and variant strains of the slime mold Dictyostelium discoideum was described. 2. 2. Anti-D. discoideum w.t. serum was found to maximally agglutinate D. discoideum w.t., Agg 73, Agg 70, and Agg 53 amoebae but failed to agglutinate Agg 91 and Agg 89 amoebae. 3. 3. Anti-Agg 73 serum effected maximal agglutination of Agg 73 and D. discoideum w.t. amoebae but failed to effect agglutination of Agg 91 or Agg 89 amoebae. 4. 4. Anti-Agg 91 serum was found to slightly agglutinate Agg 91 and Agg 89 amoebae. However, such serum failed to agglutinate D. discoideum w.t. and Agg 73 amoebae. 5. 5. Anti-Agg 89 serum proved to be similar in activity to Agg 91 serum. 6. 6. It was suggested that the failure of the aggregateless variants to complete development is associated with aberrant surface changes.

Original Text (This is the original text for your reference.)

Surface antigen defects contributing to developmental failure in aggregateless variants of the slime mold, Dictyostelium discoideum

1. 1. The procedure for obtaining antisera to wild type and variant strains of the slime mold Dictyostelium discoideum was described. 2. 2. Anti-D. discoideum w.t. serum was found to maximally agglutinate D. discoideum w.t., Agg 73, Agg 70, and Agg 53 amoebae but failed to agglutinate Agg 91 and Agg 89 amoebae. 3. 3. Anti-Agg 73 serum effected maximal agglutination of Agg 73 and D. discoideum w.t. amoebae but failed to effect agglutination of Agg 91 or Agg 89 amoebae. 4. 4. Anti-Agg 91 serum was found to slightly agglutinate Agg 91 and Agg 89 amoebae. However, such serum failed to agglutinate D. discoideum w.t. and Agg 73 amoebae. 5. 5. Anti-Agg 89 serum proved to be similar in activity to Agg 91 serum. 6. 6. It was suggested that the failure of the aggregateless variants to complete development is associated with aberrant surface changes.

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J.H. Gregg and C.W. Trygstad,.Surface antigen defects contributing to developmental failure in aggregateless variants of the slime mold, Dictyostelium discoideum. 15 (2),358-369.

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