The goal of this study was to develop a strategy for the selective destruction of cancer cells by ultrasonic irradiation in the presence of an antibody-conjugated photosensitizer. To this end, a photoimmunoconjugate (PIC) was prepared between ATX-70, a photosensitizer of a gallium-porphyrin analogue, and F11-39, a high affinity monoclonal antibody (MAb) against carcinoembryonic antigen (CEA), which is often overexpressed in various carcinoma cells. This conjugate, designated F39/ATX-70, retained immunoreactivity against purified CEA and CEA-expressing cells as determined by enzyme-linked immunosorbent assay, flow cytometry and immunofluorescence microscopic analysis. The cytotoxicity of F39/ATX-70 against CEA-expressing human gastric carcinoma cells in vitro was found to be greater than that of ATX-70 when applied in combination with ultrasound irradiation. When in vivo anti-tumor effects in a mouse xenograft model were assessed, intravenous administration of F39/ATX-70 followed by ultrasonic irradiation produced a marked growth inhibition of tumor compared with irradiation alone or irradiation after administration of ATX-70. These results suggest that the PIC between anti-CEA MAb and ATX-70 may have applications in sonodynamic therapy where destruction of CEA-expressing tumor is required.

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cells immunosorbent assay flow cytometry galliumporphyrin analogue carcinoembryonic antigen selective destruction of photoimmunoconjugate pic sonodynamic therapy ceaexpressing tumor ultrasonic irradiation anticea mab immunofluorescence microscopic atx70 growth

Hironori, Abe Motomu, Kuroki Katsuro, Tachibana Tieli, Li Aradhana, Awasthi Aruto, Ueno Hisanobu, Matsumoto Takayuki, Imakiire Yasushi, Yamauchi Hiromi, Yamada Asami, Ariyoshi Masahide, Kuroki,.Targeted sonodynamic therapy of cancer using a photosensitizer conjugated with antibody against carcinoembryonic antigen.. 22 (3),1575-80.

Hironori, Abe Motomu, Kuroki Katsuro, Tachibana Tieli, Li Aradhana, Awasthi Aruto, Ueno Hisanobu, Matsumoto Takayuki, Imakiire Yasushi, Yamauchi Hiromi, Yamada Asami, Ariyoshi Masahide, Kuroki,"Targeted sonodynamic therapy of cancer using a photosensitizer conjugated with antibody against carcinoembryonic antigen." 22

Hironori, Abe Motomu, Kuroki Katsuro, Tachibana Tieli, Li Aradhana, Awasthi Aruto, Ueno Hisanobu, Matsumoto Takayuki, Imakiire Yasushi, Yamauchi Hiromi, Yamada Asami, Ariyoshi Masahide, Kuroki,"Targeted sonodynamic therapy of cancer using a photosensitizer conjugated with antibody against carcinoembryonic antigen."