A study on the kinetics of inhibitory effect of isoferulic acid on the monophenolase and diphenolase activity of mushroom tyrosinase was carried out using enzymological kinetic analysis method in a Na2HPO4-NaH2PO4 buffer solution (pH = 6.8) at 30°C. It was found that isoferulic acid efficiently inhibits both monophenolase and diphenolase activities of mushroom tyrosinase under experimental conditions. Concentrations of isoferulic acid leading to 50% rate inhibition (IC50) on monophenolase and diphenolase activity were calculated to be 0.13 mmol/L and 0.39 mmol/L, respectively, which are much lower than that of arbutin (IC50 = 5.3 mmol/L for diphenolase activity). The presence of isoferulic acid also prolongs the lag period in the oxidation process of l-tyrosine via tyrosinase-a 4.3-min lagging was observed with the presence of 0.20 mmol/L isoferulic acid-compared to a 1.1-min lagging in the absence of isoferulic acid. The Lineweaver-Burk plot demonstrates a competitive behavior of isoferulic acid in the tyrosinase oxidation of l-3,4-dihydroxyphenylalanine, with maximum reaction rate (vm) and inhibition constant (KI) at 64.5 µM/min and 0.11 mmol/L, respectively.

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tyrosinase oxidation of l34dihydroxyphenylalanine constant tyrosinasea 43min 11min lagging enzymological kinetic analysis method monophenolase and diphenolase activity oxidation process lineweaverburk plot

Shengzhao, Gong Meijuan, Yin Zhimian, Yun,.Kinetics of inhibitory effect of isoferulic acid on mushroom tyrosinase.. 64 (4),235-41.

Shengzhao, Gong Meijuan, Yin Zhimian, Yun,"Kinetics of inhibitory effect of isoferulic acid on mushroom tyrosinase." 64

Shengzhao, Gong Meijuan, Yin Zhimian, Yun,"Kinetics of inhibitory effect of isoferulic acid on mushroom tyrosinase."